PROTEIN LADDER WESTERN BLOT BIO RAD SOFTWARE

Total Protein normalization using Stain-Free technology consistently delivers scalar linear response across a wider dynamic range. The unique characteristics of Stain-Free technology makes imaging of total protein a much better method for quantitative western blotting.įollowing a brief UV light activation, Stain-Free ﬂuorochromes are covalently bound to protein molecules in the gel, allowing them to be imaged repeatedly on the gel or on a membrane post-transfer. The use of traditional total protein stains can hinder downstream visualization of a western blot, usually requiring repetitive staining and detaining steps which is one of the obstacles preventing wider adaptation of TPN. Why is Stain-Free Total Protein Normalization Superior over Other Methods?

Accounts for variations during loading, electrophoresis, and transfer.Eliminates immuno-blotting interference associated with HKP.Enables total protein in load sample to be measured.

Total protein normalization using Stain-Free technology This technology makes it easy for researchers to adopt total protein loading control for western blotting. Stain-Free blot visualization and image acquisition require only a few seconds using a stain-free enabled imager. Stain-Free labeled proteins can be visualized in gels 1 minute after gel electrophoresis.

PROTEIN LADDER WESTERN BLOT BIO RAD SOFTWARE

An illustration of the total protein normalization feature in the Image Lab Software using a Stain-Free enabled imager.īio-Rad’s Stain-Free western blotting provides a practical, convenient, and reliable way to perform total protein normalization.

Protein ladder western blot bio rad